Antineuritic potency of synthetic and natural crystalline vitamin B1 as determined by the “bradycardia” method

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Abstract
3 specimens of crystalline aneurin hydrochloride from natural sources and one synthetic preparation all agreed in showing a potency of 25-3.0 [gamma] per 1 I.U. (This variation is within the probable exptl. error of the method: twice the standard error of the mean, under the conditions of the test, being approximately [plus or minus] 10%.) A statistical analysis of 270 separate tests with graded amounts of international standard and "unknown" indicated that the mean result will be within [plus or minus] 22% of the true value when five "tests" are made, or within [plus or minus] 16% for ten tests, [plus or minus] 12% for 20 tests, [plus or minus] 9% for 30 tests, or [plus or minus] 8% for 40 tests (each "test" means one dose given on one occasion to one rat). An appendix (by J. Wishart) confirms these calculations by an independent method of analysis.