Cellulose Acetate Electrophoresis of Creatine Phosphokinase Isoenzymes in the Diagnosis of Myocardial Infarction
Open Access
- 1 August 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 64 (2) , 209-216
- https://doi.org/10.1093/ajcp/64.2.209
Abstract
A method for rapid separation and visualization of creatine phosphokinase (EC 2.7.3.2 ATP: creatine phosphotransferase) isoenzymes on cellulose acetate has been developed. The technic uses the Beckman microzone electrophoresis system, and is applicable to other cellulose acetate systems. Incubation is with a commercially available substrate solution, and the isoenzyme bands are visualized by fluorescence using a long-wavelength ultraviolet light. An intermediate migrating isoenzyme band (CPK-MB) was detected in sera of 67 of 68 patients with myocardial infarction, and was not present in patients with other disorders that might clinically be confused with myocardial infarction.Keywords
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