Transactivation of plasmid-borne promoters by adenovirus and several herpes group viruses
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (15) , 5969-5978
- https://doi.org/10.1093/nar/12.15.5969
Abstract
This paper describes experiments to test the ability of a number of viruses of the Herpes group, and also Adenovirus-2 and SV40, to activate transcription from the Herpes simplex virus-1 glycoprotein D and the rabbit beta-globin promoters. Plasmids containing these genes were transfected into HeLa cells which were then infected with various viruses. Transcriptional activation in trans of the plasmid-borne promoters was monitored by quantitative S1 nuclease analysis of total cytoplasmic RNA isolated after infection. The results showed that Herpes simplex viruses 1 and 2, Pseudorabies virus, Variella Zoster virus, Human Cytomegalovirus, Equine herpes virus-1 and Adenovirus-2 activate transcription from both promoters tested. In contrast, SV40 did not activate transcription in trans in this assay. The possible mechanisms of this activation are discussed.Keywords
This publication has 22 references indexed in Scilit:
- Activation of a heterologous promoter in response to dexamethasone and cadmium by metallothionein gene 5′-flanking DNACell, 1984
- Transcriptional activation of cloned human β-globin genes by viral immediate-early gene productsCell, 1983
- Activation of gene expression by adenovirus and herpesvirus regulatory genes acting in trans and by a cis-acting adenovirus enhancer elementCell, 1983
- A 5′-flanking sequence essential for progesterone regulation of an ovalbumin fusion geneNature, 1983
- Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene productCell, 1982
- Mechanism of activation of early viral transcription by the adenovirus E1A gene productCell, 1981
- The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinantsNucleic Acids Research, 1981
- A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesisNature, 1980
- Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAsCell, 1979
- Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genomePublished by Elsevier ,1977