DIFFERENCE IN THE INDUCTION OF CYTOTOXIC LYMPHOCYTES BY PUBLIC ANTIGENS OF H-2b AND H-2d CELLS1

Publisher Website
Google Scholar
Abstract
SUMMARY Using the Marbrook culture system, it was established that stimulation of H-2k (CBA) lymphocytes by H-2b (C57BL/6) spleen cells induced a population of cytotoxic lymphocytes (CL) capable of lysing H-2d (P815) target cells via public antigens common to H-2b and H-2d haplotypes. In contrast, no cross-reactive lysis of H-2b (EL4) target cells was observed following stimulation by H-2d spleen cells under identical conditions. Experiments were conducted which eliminated the possibility that this non-reciprocity of cross-reaction was caused by a failure of the H-2b target, EL4, to express the common antigens. By competitive inhibition assays H-2b and H-2d spleen and tumour cells were shown to contain the common antigens in comparable quantities, and yet H-2d spleen cells failed to induce cross-reactive CL when tested at up to 1,000 times the concentration needed for induction by H-2b spleen cells. Culture conditions were altered causing a boost in cytotoxic activity and enabling the detection of a cross-reactive response induced by BALB/c (H-2d) spleen cells. However, the degree of cross-reaction observed was still weaker than that induced by C57BL/6 (H-2b) spleen cells under identical conditions. It was concluded that the common public antigens, although shown to be expressed in equivalent concentrations on the H-2b and H-2d tissues examined, were relatively poor immunogens when presented on H-2d cells (BALB/c spleen and P815 tumour), as measured by their ability to induce a clone of CL directed against them. The implications of these results for expression of receptors on CL and their precursors are discussed.

This publication has 0 references indexed in Scilit: