Codon optimization improves heterologous expression of a Schistosoma mansoni cDNA in HEK293 cells

16 February 2002

journal article
Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research

Vol. 88 (6) , 583-586
https://doi.org/10.1007/s00436-001-0585-0

Abstract

Differences in codon usage can seriously hamper the expression of cloned cDNAs in heterologous systems. In this study, we show that the expression of a cloned Schistosoma mansoni cDNA in cultured HEK293 cells was dramatically increased by rewriting a portion of the cDNA according to human preferred codon usage, suggesting that codon optimization is a valuable strategy for improving the heterologous expression of helminth sequences. We further describe a simple modification of a recursive PCR-based method, which allows the rewriting of long stretches of DNA sequence in a single PCR reaction. This method can be used to optimize the codon usage of virtually any DNA from helminths and other parasites.

Keywords

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