Temperature sensitivity of the preoptic and anterior hypothalamic neurons in organ culture.

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Abstract
Single unit activities were recorded from the preoptic and anterior hypothalamic (POAH) explants obtained from newborn mice brain to study neural temperature sensitivities during 10-34 days culture. The firing rate of recorded units was < 13 impulses/s at 35.degree. C. Two-cold sensitive, 15 warm-sensitive and 37 thermally-insensitive units were obtained. The temperature sensitivities of the cultured POAH neurons were similar to those of in vivo neurons, the discharge frequencies changed smoothly in proportion to 1.degree.-5.degree. C changes in local temperature. The thermal responses of warm-sensitive units increased after 18 days and decreased over 30 days in vitro. Two warm-sensitive neurons were identified and observed under the optical microscope. The cold-sensitive units were unable to be recorded until about 20 days in vitro. The neural temperature sensitivities of POAH neurons were maintained in the absence of the extrahypothalamic afferents.

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