Pyosemia and Carriage of Chlamydia and Ureaplasma in Infertile Men

Abstract
C. trachomatis and U. urealyticum have been implicated as causative organisms in infections involving the male and female urogenital tracts. Seminal fluid, anterior urethral swabs and 1st-voided urine specimens from men undergoing infertility evaluation, with and without pyosemia, or anterior urethritis were cultured for C. trachoamtis and U. urealyticum. The method used to isolate C. trachomatis involved cytochalasin-B treated mouse fibroblastic McCoy cells; NYC and A7 solid media were used for the isolation of U. urealyticum. Isolation of C. trachomatis from seminal fluid has not been possible even in the face of pyosemia and the presence of C. trachomatis in urine and urethral swab material. The reasons for the inability to culture C. trachomatis will be explored.