Enhanced luminescence enzyme immunoassay for factor VIII related antigen.
- 1 March 1985
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 38 (3) , 317-319
- https://doi.org/10.1136/jcp.38.3.317
Abstract
A sandwich enzyme immunoassay for [human] plasma factor VIII related antigen was developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).This publication has 10 references indexed in Scilit:
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