Amino acid sequence in lysozyme. 1. Displacement chromatography of peptides from a partial hydrolysate on ion-exchange resins

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Abstract
An attempt was made to simplify the mixture of peptides produced on partial acid hydrolysis of lysozyme through fractionation by displacement chromatography on ion-exchange resins. The peptides in the fractions so obtained were separated by 2-dimen-sional paper chromatography. The resulting peptide spots were eluted and analysed for the constituent amino acids, the N-terminal residue and, in certain cases, the C-terminal residue. The peptides whose structures are known are listed. From this information it was possible to assemble a number of probable amino acid sequences as they occur in the lysozyme molecule.