Variation of cysteine level by chlorobenzene-induced pertubation of glutathione metabolism in rat liver.

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Abstract
Effects of chlorobenzene-induced alteration in glutathione levels on cysteine metabolism in rat liver were investigated. Male Wistar rats were i.p. injected with chlorobenzene (0.2, 0.5, 1.0 or 2.0 mmol/kg body wt). Both hepatic glutathione and cysteine levels were dose-dependently decreased by the chlorobenzene 6 h after the injection. However, at 24 h, the glutathione in the rats with chlorobenzene increased significantly as compared to that in the rats without chlorobenzene. Concomitant to the elevation in glutathione levels, hepatic glutathione synthesis activities were increased by the chlorobenzene by 68-111%. No significant difference between the rats with and without chlorobenzene was observed as regards cysteine levels at 24 h. Hepatic glutamate, glycine, methionine and serine levels were unaltered but hepatic taurine levels were significantly decreased by the chlorobenzene at both 6 and 24 h. Chlorobenzene administration had no effect on hepatic cystathione synthase and cystathionase activities. A transient loss of hepatic glutathione, caused by the administration of chlorobenzene, evidently resulted in an acceleration of glutathione synthesis and an increase of cysteine demand in the liver.

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