The Estimation of Paludrine in Urine

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Abstract
A method is described for the rapid estimation of paludrine in urine based on the extraction of the benzene soluble copper complex supplemented by a color development with Na diethyldithio-carbonate. Urine (2 ml.), copper reagent (1 ml.) and caustic soda (1 ml.) are pipetted into a tube and allowed to stand a few minutes. Benzene (5 ml.) is added and shaken for 2 mins. The benzene is decanted, washed, and shaken with 1 ml. of Na diethyldithiocarbonate for 1 min. The intensity of the golden-yellow color is estimated in a Spekker absorptiometer using Ilford violet 601 filters and the amt. of paludrine read from a standard curve.

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