USE OF SORBIC ACID ENRICHMENT MEDIA FOR SPECIES OF CLOSTRIDIUM

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Abstract
It was found that the 0.12% sorbic acid liver infusion medium was quite satisfactory for the growth of 47 cultures representing 20 spp. and types of the genus Clostridium. Considerable variation in resistance to sorbic acid was observed with the different species. Some cultures of the most tolerant spp. including C. parabotulinum, C. acetobutylicum, C. felsineum, C. tetani, and C. thermosaccharolyticum grew in the presence of 3.0% sorbic acid. The limiting pH for growth of the different species in the acid range was not appreciably affected by the presence of 0.12% sorbic acid in liver infusion without phosphate. The various spp. sporulated well, and their spores germinated without noticeable delay in the 0.12% sorbic acid medium if the pH value was in the required range. Sorbic acid is utilized as a C source by some spp.

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