Characterization of a novel zinc binding site of protein kinase C inhibitor‐1
Open Access
- 11 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (1) , 14-18
- https://doi.org/10.1016/0014-5793(91)80238-x
Abstract
The zinc‐binding properties of an endogenous protein inhibitor of protein kinase C was studied. Equilibrium gel penetration revealed that 1 mol of this protein binds 0.97 mol of zinc with a dissociation constant of 4.3 μM. The site of zinc‐binding. MVVNEGSDGGQSVYHVHLHVLGGR, was indentified by a multi‐step process consisting of tryptic digestion, fragment isolation, transfer to nitrocellulose, and hybridization with 65ZnCl2. Binding of 65ZnCl2 to selected synthetic fragments further localized the site of interaction to the sequence QSVYHVHLHVL. This region contains 3 closely positioned histidine residues and represent a novel zinc‐binding site.Keywords
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