Enzyme Electrode Sensor for Hydrogen Peroxide

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Abstract
An enzymatic sensor was constructed from an oxygen amperometric sensor on whose surface a dialysis membrane containing covalently bonded catalase was set. Immobilization of the enzyme on the dialysis membrane surface was achieved with 2,4-dichloro-6-methoxy-s-triazine, a derivative of cyanuric chloride, which unlike others of its monosubstituted derivatives ensures some advantages. The time of measurement is less than 12 sec, for the kinetic method of the initial slope and one minute for the steady-state method. The sensor responds linearly to hydrogen peroxide in the concentration range 10−3 - 10−5 moles/1. The utilization of this sensor in intermittent and continuous operation in a laboratory enzymatic minireactor is discussed.