Comparison of Vibrio parahaemolyticus hemolysin (Vp-TRH) produced by environmental and clinical isolates

15 April 1992

journal article
research article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 92 (2) , 157-161
https://doi.org/10.1016/0378-1097(92)90505-i

Abstract

TDH-related hemolysin (Vp-TRH) produced by Kanagawa-phenomenon-negative (KP⁻) Vibrio parahaemolyticus has been demonstrated to be a possible virulence determinant. Though almost half of KP⁻ isolates examined from diarrhoeal patients produced Vp-TRH, few reports mentioned the ability of environmental isolates to produce Vp-TRH. Considering the route of infection with V. parahaemolyticus, this toxin must be produced by the organisms in the sea or in sea food. To confirm that Vp-TRH produced by V. parahaemolyticus could be involved in sea-food-borne diarrhoeas, Vp-TRH-producing strains were isolated from the environment, identified and hemolysin purified from these strains was compared to hemolysin (Vp-TRH) isolated from diarrhoeal patients. The results showed that the hemolytic activity, antigenicity, reactivity in the rabbit ileal loop test and N-terminal amino acid sequence of Vp-TRH from environmental strains was indistinguishable from the toxin of clinical origin.

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