Lytic Activity of Recombinant Bacteriophage φ11 and φ12 Endolysins on Whole Cells and Biofilms of Staphylococcus aureus
- 1 January 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (1) , 347-352
- https://doi.org/10.1128/aem.01616-06
Abstract
The recombinant phi11 endolysin hydrolyzed heat-killed staphylococci as well as staphylococcal biofilms. Cell wall targeting appeared to be a prerequisite for lysis of whole cells, and the combined action of the endopeptidase and amidase domains was necessary for maximum activity. In contrast, the phi12 endolysin was inactive and caused aggregation of the cells.Keywords
This publication has 38 references indexed in Scilit:
- Therapeutic Effects of Bacteriophage Cpl-1 Lysin against Streptococcus pneumoniae Endocarditis in RatsAntimicrobial Agents and Chemotherapy, 2005
- Use of Confocal Microscopy To Analyze the Rate of Vancomycin Penetration through Staphylococcus aureus BiofilmsAntimicrobial Agents and Chemotherapy, 2005
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- Guidelines for the Prevention of Intravascular Catheter-Related InfectionsPublished by American Academy of Pediatrics (AAP) ,2002
- Comparative analysis of the genomes of the temperate bacteriophages φ11, φ12 and φ13 of Staphylococcus aureus 8325Gene, 2002
- Mechanisms of antibiotic resistance in bacterial biofilmsInternational Journal of Medical Microbiology, 2002
- Role of fibronectin in staphylococcal adhesion to metallic surfaces used as models of orthopaedic devicesJournal of Orthopaedic Research, 1994
- Growth-rate-independent killing by ciprofloxacin of biofilm-derived Staphylococcus epidermidis evidence for cell-cycle dependencyJournal of Antimicrobial Chemotherapy, 1992
- Bacterial Biofilms in Nature and DiseaseAnnual Review of Microbiology, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985