The Protein Nature of the Thy-1.2 Alloantigen as Expressed by the Murine Lymphoblastoid Line S-49.1 TB·2·3
Open Access
- 1 September 1975
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 115 (3) , 626-630
- https://doi.org/10.4049/jimmunol.115.3.626
Abstract
This preliminary study was undertaken to investigate the chemical nature of the Thy-1.2 antigen expressed on the murine cell line S-49.1 TB·2·3 (S-49). The presence of the Thy-1.2 antigen was indicated by the inhibition of AKR anti-C3-Thy-1.2 serum induced lysis of 51Cr-primed target cells. It was found that limited digestion of S-49 cells with crude papain yielded a Thy-1.2-containing solution. The protein nature of the Thy-1.2 antigen obtained in this manner was indicated by changes after proteolytic digestion. Separate digestions with crystalline papain, insolubilized papain, and insolubilized protease all destroyed the Thy-1.2 activity. These results suggest that the protein moiety is necessary for Thy-1.2 activity.Keywords
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