Glutamine synthetases of Corynebacterium glutamicum: transcriptional control and regulation of activity
Open Access
- 10 July 2001
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 201 (1) , 91-98
- https://doi.org/10.1016/s0378-1097(01)00244-0
Abstract
Regulation of glnA expression and glutamine synthetase I activity was analyzed in Corynebacterium glutamicum . Transcription is regulated by the global repressor protein AmtR, essential for derepression of glnA transcription are GlnK and uridylyltransferase, key proteins of the C. glutamicum nitrogen regulatory system. Glutamine synthetase I activity is controlled by adenylylation/deadenylylation via adenylyltransferase. The gene encoding this bifunctional enzyme, glnE , was isolated and its function was characterized by deletion analysis. Upstream of glnE , a second gene encoding a GSI-type protein in C. glutamicum was isolated. This gene, designated glnA2 , forms an operon with glnE , its transcription is not regulated and neither its deletion or overexpression showed any effect. Therefore, the physiological role of glnA2 remains unclear.Keywords
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