Isolation of a developmental gene of Bacillus subtilis and its expression in Escherichia coli.

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Abstract
Glucose dehydrogenase of B. subtilis is a developmental enzyme that is not found in growing (vegetative) cells but is synthesized after the differentiation process that leads to the production of endospores has started. The gene coding for this enzyme from a .lambda. Charon 4A phage library of B. subtilis DNA. It is transcribed and translated in vegetative cells of the nondifferentiating organism E. coli into enzymatically active glucose dehydrogenase that has the same physicochemical properties as the enzyme produced in B. subtilis during sporulation. Subcloning of the .lambda. DNA insert into plasmid pBR322 derivatives showed that the glucose dehydrogenase gene was transcribed in E. coli from a promoter within the B. subtilis genome.