The block of the expressed L‐type calcium channel is modulated by the β3 subunit
Open Access
- 9 October 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 373 (2) , 103-107
- https://doi.org/10.1016/0014-5793(95)01013-5
Abstract
The α 1C subunit of the L‐type calcium channel was stable, expressed alone or in combination with the β 3 subunit in Chinese hamster ovary cells. The β 3 subunit enhanced significantly the inactivation of barium currents indicating that both subunits interacted with each other. The β 3 subunit decreased signinicantly the half‐maximal inhibitory concentration of the calcium channel blockers (−)‐gallopamil and verapamil, but did not affect significantly the block caused by isradipine and mibefradil at the holding potentials of −80 mV and −40 mV. These results suggest that the β 3 subunit affects distinctly the interaction of the expressed α 1c subunit with different classes of organic calcium channel blockers.Keywords
This publication has 24 references indexed in Scilit:
- Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits.The Journal of general physiology, 1995
- Calcium channels: The β‐subunit increases the affinity of dihydropyridine and Ca2+ binding sites of the α1‐subunitFEBS Letters, 1994
- Calcium channel β-subunit binds to a conserved motif in the I–II cytoplasmic linker of the α1-subunitNature, 1994
- Molecular Basis for CA2+ Channel DiversityAnnual Review of Neuroscience, 1994
- Subunit-dependent modulation of recombinant L-type calcium channels. Molecular basis for dihydropyridine tissue selectivity.Circulation Research, 1993
- Potentiation by the β Subunit of the Ratio of the Ionic Current to the Charge Movement in the Cardiac Calcium ChannelScience, 1993
- Functional expression of a rapidly inactivating neuronal calcium channelNature, 1993
- Receptor sites for Ca2+ channel antagonistsTrends in Pharmacological Sciences, 1992
- Tissue‐specific expression of high‐voltage‐activated dihydropyridine‐sensitive L‐type calcium channelsEuropean Journal of Biochemistry, 1991
- Voltage-dependent block of calcium channel current in the calf cardiac Purkinje fiber by dihydropyridine calcium channel antagonists.Circulation Research, 1984