DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes
Top Cited Papers
- 7 August 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (17) , 10954-10957
- https://doi.org/10.1073/pnas.162375999
Abstract
The light-harvesting properties of cationic conjugated polymers are used to sensitize the emission of a dye on a specific peptide nucleic acid (PNA) sequence for the purpose of homogeneous, "real-time" DNA detection. Signal transduction is controlled by hybridization of the neutral PNA probe and the negative DNA target. Electrostatic interactions bring the hybrid complex and cationic polymer within distances required for Förster energy transfer. Conjugated polymer excitation provides fluorescein emission >25 times higher than that obtained by exciting the dye, allowing detection of target DNA at concentrations of 10 pM with a standard fluorometer. A simple and highly sensitive assay with optical amplification that uses the improved hybridization behavior of PNA/DNA complexes is thus demonstrated.Keywords
This publication has 31 references indexed in Scilit:
- A fluorescent complex between ethidium bromide and nucleic acids: Physical—Chemical characterizationPublished by Elsevier ,2004
- Energy Transfer in Mixtures of Water- Soluble Oligomers: Effect of Charge, Aggregation, and Surfactant ComplexationAdvanced Materials, 2002
- PNA for rapid microbiologyJournal of Microbiological Methods, 2001
- Tumor targeting with surface-shielded ligand–polycation DNA complexesJournal of Controlled Release, 2001
- Electronic Detection of Nucleic AcidsThe Journal of Molecular Diagnostics, 2001
- Recognition of DNA Topology in Reactions between Plasmid DNA and Cationic CopolymersJournal of the American Chemical Society, 2000
- Photoluminescence of Water-Soluble Conjugated Polymers: Origin of Enhanced Quenching by Charge TransferMacromolecules, 2000
- Molecular Recognition of PNA-Containing Hybrids: Spontaneous Assembly of Helical Cyanine Dye Aggregates on PNA TemplatesJournal of the American Chemical Society, 1999
- Chain Length Recognition: Core-Shell Supramolecular Assembly from Oppositely Charged Block CopolymersScience, 1999
- PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rulesNature, 1993