Roles of Mg2+ in TPP‐dependent riboswitch
- 8 April 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (12) , 2583-2588
- https://doi.org/10.1016/j.febslet.2005.03.074
Abstract
We quantified the effect of Mg(2+) on thiamine pyrophosphate (TPP) binding to TPP-dependent thiA riboswitch RNA. The association constant of TPP binding to the riboswitch at 20 degrees C increased from 1.2 x 10(6) to 50 x 10(6) M(-1) as the Mg(2+) concentration increased from 0 to 1 mM. Furthermore, circular dichroic spectra under various conditions showed that 1 mM Mg(2+) induced a local structural change of the riboswitch, which might be pivotal for TPP binding. These results indicate that a physiological concentration of Mg(2+) can regulate TPP binding to the thiA riboswitch.Keywords
This publication has 36 references indexed in Scilit:
- Structural Basis for Discriminative Regulation of Gene Expression by Adenine- and Guanine-Sensing mRNAsChemistry & Biology, 2004
- Structural Competition Involving G-Quadruplex DNA and Its ComplementBiochemistry, 2003
- Immobilized Small Deoxyribozyme to Distinguish RNA Secondary StructuresBiochemistry, 2002
- Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysateThe EMBO Journal, 2001
- Stabilization of RNA tertiary structure by monovalent cationsJournal of Molecular Biology, 2000
- tRNA mimicsCurrent Opinion in Structural Biology, 1998
- Site‐specific cleavage reaction catalyzed by leadzyme is enhanced by combined effect of lead and rare earth ionsFEBS Letters, 1996
- Fluorescence-Detected Stopped Flow with a Pyrene Labeled Substrate Reveals That Guanosine Facilitates Docking of the 5' Cleavage Site into a High Free Energy Binding Mode in the Tetrahymena RibozymeBiochemistry, 1994
- Kinetics for reaction of a circularized intervening sequence with CU, UCU, CUCU, and CUCUCU: mechanistic implications from the dependence on temperature and on oligomer and magnesium concentrationsBiochemistry, 1988
- Circular dichroism studies on the conformation of transfer ribonucleic acid in the presence of different divalent cationsBiochemistry, 1973