Sensitivity of different human cell lines in the biologic evaluation of dental resin‐based restorative materials
- 1 February 1981
- journal article
- research article
- Published by Wiley in European Journal of Oral Sciences
- Vol. 89 (1) , 102-107
- https://doi.org/10.1111/j.1600-0722.1981.tb01283.x
Abstract
Three different cell lines of human origin, i.e., human epithelial cells (NCTC 2544 [adult human male skin epithelium cells]) and epithelial- and fibroblast-like cells from human gingiva, were tested for their sensitivity to 3 different dental resin-based restorative materials using 4 different assay techniques. The techniques applied were cell growth measurements by cell number, 51Cr-release, agar overlay with neutral red staining and colony formation. The ranking of the cells according to their sensitivity to the materials varied with the assay technique applied, with no cell line being consistently more sensitive than the others. The type of medium applied can apparently be important for the results obtained.Keywords
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