Calcium chloride effects on nuclear development in Tetrahymena

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Abstract
SUMMARY: Conjugating pairs of syngen 1 exposed to 0·1 M-CaCl2 during the time of macronuclear development often abort the new macronuclei and retain the old macronucleus and its associated phenotypes. The new micro-nucleus is retained, however, so that macro–micro heterocaryons can be constructed. Because the macronucleus is from a sexually mature strain, the heterocaryon is capable of mating immediately, without passing a period of sexual immaturity. Macronuclear abortion may prove a useful short-cut in genetic analysis.