Analysis of Theophylline in Serum and Whole Blood Samples by High-Pressure Liquid Chromatography

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Abstract
A high-pressure liquid chromatographic procedure for theophylline, which can be applied to both ante-mortem and post-mortem samples, is described. The procedure routinely requires a 250µl sample but as little as 50µl of serum can be analyzed. Analytical results obtained using the described high-pressure liquid chromatographic procedure are compared with those obtained using a spectrophotometric method. In addition, whole-blood and sera specimens collected at the same time were analyzed and the results compared. Good correlation was observed between the high-pressure liquid chromatographic and spectrophotometric method. It was also found that the results obtained from the analysis of whole blood were comparable to the serum theophylline concentrations.