Chemiluminescent Enzyme Immunoassay A Review

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Abstract
Highly sensitive chemiluminescent enzyme immunoassays developed in our laboratory are reviewed. Oxidases, peroxidase and glucose oxidase, generating H2O2 can be assayed by isoluminol/microperoxidase or peroxyoxalate/fluorescent dye. β-D-Galactosidase is also assayed by chemiluminescence reaction based on the coupled enzyme reaction using lactose/glucose oxidase. β-D-Galactosidase can be measured by using o-nitrophenyl β-D-galactoside as substrate and chemiluminescence reaction of NADH after coupled enzyme reaction of galactose dehydrogenase. Sucrose and lucigenin are used as substrate and chemiluminescent reagent for the assay of invertase. Glucose-6-phosphate is determined by using glucose 6-phosphate as substrate and chemiluminescent assay of NADH. Alkaline phosphatase is assayed by using NADP+, alcohol dehydrogenase and hemiluminescence reaction of NADH. These methods can be applied successfully to the chemiluminescent enzyme immunoassays for various hormones and drugs in biological fluids.

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