Tritium Labelled 1‐O‐Octadecyl‐2‐O‐methyl‐rac‐glycero‐3‐phosphocholine (ALP) and 1‐S‐Hexadecyl‐2‐O‐ethyl‐rac‐thioglycero‐3‐phosphocholine (Thio‐ALP)

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Abstract
The phospholipids, ALP and Thio‐ALP, are non‐hydrolyzable analogues of platelet activating factor (PAF). Interest in ALP and thio‐ALP centers upon their activity as potential antineo‐plastic agents. A variety of mechanisms of action have been attributed to these compounds including inhibition of a phospholipid cofactor of a phospholipid sensitive Ca+2‐dependent protein kinase. Thio‐ALP is at least as active as a growth inhibitor of the HL‐60 promyelocytic leukemic cell line as is ALP‐the most active reference analogue reported in the literature and is approximately twice as active against the BG‐1 and BG‐3 human ovarian carcinoma cell lines. To aid in further biochemical studies, we report the synthesis of high specific activity tritium labelled ALP and thio‐ALP. These products were obtained by palladium catalyzed reduction of 1‐0‐octadecenyl and l‐S‐hexadecenyl precursors with carri'er‐free tritium gas.