Biochemical Basis of Different Sensitivity to Methotrexate inDaucus carotaandOryza sativaCell Cultures
- 1 September 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 34 (9) , 1189-1195
- https://doi.org/10.1093/jxb/34.9.1189
Abstract
The biochemical basis of the different sensitivity to methotrexate of Daucus carota and Oryza sativa cell cultures has been investigated. Carrot cells have a dihydrofolate reductase activity about ten times higher than rice cells. In addition, they show a lower uptake rate of the inhibitor. No relevant differences have been found in the Km value for the dihydrofolate of the two enzymes and in the degree of inhibition of their activity by methotrexate.Keywords
This publication has 2 references indexed in Scilit:
- Effect of Folate Analogues on the Activity of Dihydrofolate Reductases and on the Growth of Plant OrganismsJournal of Experimental Botany, 1981
- Regulation of folate reductase synthesis in sensitive and methotrexate-resistant sarcoma 180 cells. In vitro translation and characterization of folate reductase mRNA.Journal of Biological Chemistry, 1976