Radioimmunological Measurement of Beta-Endorphin in Equine Plasma
- 1 July 1983
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 173 (3) , 454-459
- https://doi.org/10.3181/00379727-173-41670
Abstract
Radioimmunoassay procedures were developed and validated for the quantification of .beta.-endorphin (.beta.-EP)-like immunoreactivity in equine plasma. .beta.-EP could be quantitatively extracted from plasma with silicic acid powder and subsequently assayed; however, valid estimates of this hormone could also be obtained on unextracted plasma. Although .beta.-lipotropin (.beta.-LPH) cross-reacted in the assay, it was not necessary to correct for .beta.-LPH activity when assaying unextracted plasma because chromatographic analyses showed that 92% of the immunoreactivity in plasma extracts was similar in MW to authentic .beta.-EP (1-31). In addition electroacupuncture treatment did not alter the relative proportion of immunoreactivity among different MW fractions.This publication has 4 references indexed in Scilit:
- Relationship between plasma concentrations of immunoreactive beta-endorphin and food intake in ratsPeptides, 1983
- Equine Cushing's Disease: Plasma Immunoreactive Proopiolipomelanocortin Peptide and Cortisol Levels Basally and in Response to Diagnostic Tests*Endocrinology, 1982
- Proopiolipomelanocortin Peptides in Normal Pituitary, Pituitary Tumor, and Plasma of Normal and Cushing's Horses*Endocrinology, 1982
- β-endorphin immunoreactivity in rat and human blood: Radioimmunoassay, comparative levels and physiological alterationsLife Sciences, 1979