DNA Dependent RNA Polymerase from Ehrlich Ascites Tumor Cells

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Abstract
A protein was isolated which repressed RNA polymerase II [EC 2.7.7.6] of Ehrlich ascites tumor cells. This protein, named R protein, was only inhibitory when native DNA was used as template with homologous RNA polymerase. This protein had affinity for DNA. It was inhibitory when added at the initiation stage of transcription, but not when added once transcription had started. Its molecular weight was estimated as 38,000 daltons by polyacrylamide gel electrophoresis containing sodium dodecylsulfate. The R protein did not affect the size of RNA synthesized in vitro. Studies on its effect on RNA synthesis in the presence of S-II, a factor stimulating RNA polymerase II of Ehrlich ascites tumor cells, suggested that R protein preferentially repressed synthesis of a certain species of RNA which is synthesized in the presence of S-II. The molecular mechanism of the inhibition of RNA synthesis by this protein is discussed.

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