Effects of a new pyrazolo [3, 4-d]pyrimidine on growth and morphology of Candida albicans

Abstract
A new pyrazolo [3, 4-d]pyrimidine derivative was synthesized and its antifungal activity evaluated in vitro against mycelial and yeast cells of Candida albicans. The most striking ultrastructural changes following treatment with 10–30 μg/ml (mycelia) and 25–75 μg/ml (yeasts) consisted in the deterioration of the organelle membranes and in aberrant thickenings of the cell wall. The complete disorganization of the cytoplasmic structures seemed to be the final event.