Influence of Chain Length and Unsaturation on the Effects of Fatty Acids on Phosphoglyceride Biosynthesis in Isolated Rat and Pig Hepatocytes
- 1 March 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 63 (1) , 65-70
- https://doi.org/10.1111/j.1432-1033.1976.tb10207.x
Abstract
Hepatocytes isolated from rat or pig by collagenase perfusion were incubated with [3H]glcyerol and different albumin-bount fatty acids. Among C22 fatty acids docosahexaenoic acid stimulated phosphatidylethanolamine synthesis in rat hepatocytes most effectively. Addition of docosahexaenoic acid plus either palmitic or stearic acid resulted almost in the same stimulation whereas combinations of this acid with lauric or myristic acid had no effect. Lauric acid and myristic acid alone inhibited phosphatidylethanolamine synthesis. The chain length specificity for monoenoic fatty acids was similar, the hexadecenoic and octadecenoic acids (both cis and trans) being most stimulatory. The addition of 0.2 mM ethanolamine markedly stimulated phosphatidylethanolamine synthesis, but most effects of fatty acids were similar in its presence or absence.Keywords
This publication has 25 references indexed in Scilit:
- Dispersion of viable pig liver cells with collagenaseLife Sciences, 1975
- Substrate-selectivity of rat liver microsomal 1,2-diacylglycerol:CDPcholine(ethanolamine) choline(ethanolamine)phosphotransferase in utilizing endogenous substratesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- The binding of long chain fatty acid CoA to Z, a cytoplasmic protein present in liver and other tissues of the ratBiochemical and Biophysical Research Communications, 1974
- Influence of chain length and degree of unsaturation on plasma free fatty acid uptake by the perfused rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Biochemistry of Bacterial MembranesAnnual Review of Biochemistry, 1973
- Biosynthesis of rat liver phosphatidylethanolamines from intraportally injected ethanolamineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- The binding of fatty acids to cytoplasmic proteins: Binding to Z protein in liver and other tissues of the ratBiochemical and Biophysical Research Communications, 1972
- The effect of dietary fat on the molecular species of lecithin from rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1966
- The effects of trans, trans-linoleate upon the metabolism of linoleate and linolenate and the positional distribution of linoleate isomers in liver lecithinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
- The fatty acids of individual liver phosphatides from rats grown on a fat-free dietBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965