IMMUNOLOGICAL RESPONSES TO COMPLEX SALTS OF PLATINUM .1. SPECIFIC IGE ANTIBODY-PRODUCTION IN THE RAT

  • 1 January 1984
    • journal article
    • research article
    • Vol. 57  (1) , 107-114
Abstract
Ammonium tetrachloroplatinate II ([NH4]2PtCl4) was used in free and conjugated forms with ovalbumin in an attempt to elicit specific antibody directed against either the free Pt salt or the Pa moiety of ovalbumin-Pt conjugates in the hooded Lister rat. Immunization with free Pt salt via i.p., i.m., intradermal, s.c., intratracheal and footpad routes over a wide range of doses (1 .mu.g-1 mg) employing both [Bordetella] pertussis and/or AlCOH3 gel as adjuvants failed to induce specific IgE antibody, either primary or secondary, as shown by direct skin, PCA [passive cutaneous anaphylaxis] test or RAST [radioallergosorbent test]. Conjugation of (NH4)2 PtCl4 with ovalbumin produced conjugates, with between 2 and 10 haptenic Pt groups/ovalbumin molecule, capable of inducing IgE antibody directed against the Pt moiety as determined by heterologous PCA challenge, where carrier cross-reactivity was excluded, and by specific RAST, confirmed by RAST inhibition.