Novel Adeno-Associated Virus Vector Vaccine Restricts Replication of Simian Immunodeficiency Virus in Macaques
Open Access
- 15 January 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (2) , 955-965
- https://doi.org/10.1128/jvi.79.2.955-965.2005
Abstract
Gene transfer vectors based on recombinant adeno-associated virus (rAAV) are simple, versatile, and safe. While the conventional applications for rAAV vectors have focused on delivery of therapeutic genes, we have developed the system for delivery of vaccine antigens. In particular, we are interested in generating rAAV vectors for use as a prophylactic human immunodeficiency virus type 1 (HIV-1) vaccine. To that end, we constructed vaccine vectors that expressed genes from the simian immunodeficiency virus (SIV) for evaluation in the monkey SIV model. After a single intramuscular dose, rAAV/SIV vaccines elicited SIV-specific T cells and antibodies in macaques. Furthermore, immunized animals were able to significantly restrict replication of a live, virulent SIV challenge. These data suggest that rAAV vaccine vectors induced biologically relevant immune responses, and thus, warrant continued development as a viable HIV-1 vaccine candidate.Keywords
This publication has 147 references indexed in Scilit:
- Induction of HIV‐specific antibody response and protection against vaginal SHIV transmission by intranasal immunization with inactivated SHIV‐capturing nanospheres in macaquesJournal of Medical Virology, 2004
- Autoimmune anemia in macaques following erythropoietin gene therapyBlood, 2004
- Erythropoietin gene therapy leads to autoimmune anemia in macaquesBlood, 2004
- Major Histocompatibility Complex Class I Alleles Associated with Slow Simian Immunodeficiency Virus Disease Progression Bind Epitopes Recognized by Dominant Acute-Phase Cytotoxic-T-Lymphocyte ResponsesJournal of Virology, 2003
- Chromosomal effects of adeno-associated virus vector integrationNature Genetics, 2002
- An IL-2/Ig Fusion Protein Influences CD4+T Lymphocytes in Naive and Simian Immunodeficiency Virus-Infected Rhesus MonkeysAIDS Research and Human Retroviruses, 2001
- Vaccine-Elicited V3 Loop-Specific Antibodies in Rhesus Monkeys and Control of a Simian-Human Immunodeficiency Virus Expressing a Primary Patient Human Immunodeficiency Virus Type 1 Isolate EnvelopeJournal of Virology, 2001
- In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIVSF33A: Correlation to in vivo outcomeJournal of Medical Primatology, 1998
- Cell Lines for the Production of Recombinant Adeno-Associated VirusHuman Gene Therapy, 1995
- Plasmid Integration, Amplification and Cytogenetics in CHO Cells: Questions and CommentsBiologicals, 1994