Host-controlled modification and restriction in Bacillus subtilis

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Abstract
A Bsu168-specific restriction deficient (r 168 - ) mutant of Bacillus subtilis Marburg 168 was transformed to be BsuR-specific restriction proficient (r R + ) with B. subtilis R DNA as efficiently as the Bsu168-specific restriction proficient (r 168 + ) parental strain (hsrM+, hsdR-). We constructed r R + m R + r 168 + m 168 + strain (ISMR 4), r R + m R + r 168 - m 168 + strain (ISR 11) and r R + m R + r 168 - m 168 - strain (ISR 6) from strain 101 (r 168 + m 168 + ), strain 1012 (r 168 - m 168 + ) and strain RM125 (r 168 - m 168 - ), respectively by transformation with B. subtilis R DNA, and tested their restriction and modification activities on phage ϕ105C. The results show that the sites recognized by Bsu168-specific restriction and modification enzymes and the sites recognized by BsuR-specific ones are not overlapping. We conclude that the Bsu168-modification and restriction system and the BsuR-modification and restriction system are controlled independently by two distinct sets of genes in the r R + m R + transformant of r 168 + m 168 + strain B. subtilis 168.

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