The Role of Escherichia coli dnaG Function in Coliphage M 13 DNA Synthesis

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Abstract
Examination of the role of Excherichia coli dnaG function in different stages of M 13 phage DNA synthesis by ultracenuifugal analysis of intracellular phage DNA in a thermosensitive dnaG mutant shows that: (a) the formation of parental double‐strand replicative‐form DNA (rfDNA) from the infecting virus is independent of dnaG function; (b) the synthesis of progeny rlDNA requires dnaG product: (c) after a pool of rfDNA is made up, dnaG function is not required for the progeny single strand DNA (ssDNA) synthesis. The ssDNAs produced under nonpermissive condition are mostly circular and biologically functional.