Glucose Transport inVitis viniferaL. Protoplasts

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Abstract
Transport of glucose and its analogues was studied in grapevine (Viris vinfera L. cv. Soultanina) leaf protoplasts. The transport system was hexose specific and the stereospecificity was closely related to carbon-1 of the glucose molecule. Glucose structural analogues were not metabolized beyond the stage of phosphorylation and differences between these compounds and glucose were observed in their transport rates and in their specificity for the carrier. Concentration-dependent uptake of labelled glucose by grapevine protoplasts was linear for concentrations higher than 1·5 mol m−3 at lower concentrations a saturating pattern was observed. The carrier was driven by the proton motive force and the substrate entered the cell probably in an unchanged form. Efflux studies were not useful as an indication of the rate of metabolism or assimilation of transported compounds in grapevine protoplasts.

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