Spin-label electron spin resonance study of bacteriophage M13 coat protein incorporation into mixed lipid bilayers

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Abstract
The major coat protein of bacteriophage M13 was incorporated in mixed dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (80/20 w/w) vesicles probed with different spin-labeled phospholipids, labeled on the C-14 atom of the sn-2 chain. The specificity for a series of phospholipids was determined from a motionally restricted component seen in the electron spin resonance (ESR) spectra of vesicles with the coat protein incorporated. At 30 .degree.C and pH 8, the fraction of motionally restricted phosphatidic acid spin-label is 0.36, 0.52, and 0.72 for lipid/protein ratios of 18, 14, and 9 mol/mol, respectively. The ESR spectra, analyzed by digital subtraction, resulted in a phospholipid preference following the pattern cardiolipin = phosphatidic acid < stearic acid = phosphatidylserine =phosphatidylglycerol > phosphatidylcholine = phosphatidylethanolamine. The specificities found are related to the composition of the target Escherichi coli cytoplasmic membrane.

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