Detection by polyacrylamide gel electrophoresis of the human serum amyloid A protein in inflammatory sera
- 1 January 1987
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 8 (10) , 499-501
- https://doi.org/10.1002/elps.1150081010
Abstract
Human serum amyloid A protein in inflammatory sera is easily identified by simple protein staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis as the lowest molecular weight detected proteic band. Western blotting followed by immunoblot has been used to demonstrate this result. By contrast, serum amyloid A protein is not detectable by these combined techniques in normal sera.This publication has 8 references indexed in Scilit:
- Blotting methods applied to investigations of the mitogenic activity of phytohaemagglutinin (PHA)Journal of Immunological Methods, 1985
- Glycosphingolipid-blotting: an immunological detection procedure after separation by thin layer chromatographyJournal of Immunological Methods, 1984
- MECHANISM OF REDUCED AMYLOID-A-DEGRADING ACTIVITY IN SERUM OF PATIENTS WITH SECONDARY AMYLOIDOSISThe Lancet, 1982
- Properties of Reference Escherichia coli Endotoxin and Its Phthalylated Derivative in HumansThe Journal of Infectious Diseases, 1981
- Isolation and characterization of the amyloid-related apoprotein (SAA) from human high density lipoprotein.Proceedings of the National Academy of Sciences, 1980
- Amyloid protein SAA is associated with high density lipoprotein from human serum.Proceedings of the National Academy of Sciences, 1977
- Amyloid-Related Serum Protein SAA from Three Animal Species: Comparison with Human SAAThe Journal of Immunology, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970