Preservation of Channel Catfish Sperm
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Transactions of the American Fisheries Society
- Vol. 105 (3) , 469-474
- https://doi.org/10.1577/1548-8659(1976)105<469:poccs>2.0.co;2
Abstract
Three activators, 8 extenders and 3 protective agents at 2 concentration levels each were used to determine the best combination that will maintain in storage the viability of channel catfish (Ictalurus punctatus) sperm cells. There was a highly significant (P < 0.01) difference in motility score among activators. Saline (0.65%) gave the highest motility score. Extended sperm cells became motile when activated after 2 mo. of storage at 4.degree. C. Extender I (Truscott and Ilder''s Hfx no. 1) with 10% dimethyl sulfoxide (DMSO) and 2 h equilibration produced the highest motility scores after freezing of 24 h or 1 wk. Freezing rate was controlled by placing vials of sperm in a double-layered, kapok-lined paper bag and freezing at the height of 127 mm above the liquid N level.This publication has 5 references indexed in Scilit:
- Sub-Zero Preservation of Atlantic Salmon SpermJournal of the Fisheries Research Board of Canada, 1968
- The Viability of Trout Germ Cells Immersed in WaterThe Progressive Fish-Culturist, 1966
- Low temperature research on spermatozoa and eggsCryobiology, 1964
- The Freezing Point Depression of Bull Semen and Its Relation to the Diluter ProblemJournal of Animal Science, 1948
- The motility and viability of rabbit spermatozoa at different hydrogen‐ion concentrationsThe Journal of Physiology, 1947