Studies on the Response of Carrot Cells to aSclerotinia sclerotiorumElicitor: Induction of the Expression of an Extracellular Glycoprotein mRNA

1 January 1996

journal article
Published by Scientific Societies in Molecular Plant-Microbe Interactions®

Vol. 9 (7) , 658-63
https://doi.org/10.1094/mpmi-9-0658

Abstract

A heat-released elicitor (HRE) obtained from Sclerotinia sclerotiorum mycelium induced a transient increase in the activity of phenylalanine-ammonia lyase (PAL) in a carrot cell culture. Differential display reactions carried out on total RNA extracted from cells exposed for 7 h to the HRE revealed a complex cell response involving the induction and repression of several mRNAs. One of these elicitor-induced mRNAs encodes a 42.6-kDa protein. Northern (RNA) blot analysis and reverse-transcription polymerase chain reaction studies showed that noninduced cells have a basal expression of the 42.6-kDa protein mRNA that was stimulated five- to 10-fold by treatment with the elicitor.

Keywords

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