RHODAMINE AS A FLUORESCENT-PROBE OF LYMPHOCYTE-ACTIVATION

  • 1 January 1979
    • journal article
    • research article
    • Vol. 36  (2) , 235-240
Abstract
Fresh rat and mouse lymphoid cells were labeled by stable linkage with tetramethylrhodamine isothiocyanate (TMRITC). An increase or decrease of the fluorescent emission of the cells, detected by microfluorimetry, was induced by mitogen [phytohemagglutin, pokeweed mitogen or concanavalin A] stimulation or the mixed lymphocyte reaction. The change in fluorescence was observed within 3 h of mitogen stimulation and within 0.5 h in the mixed lymphocyte test. These early cellular responses were detectable consistently whether the labeling was done before or after mitogen stimulation; post-labeling only was studied in the mixed lymphocyte reaction. The method is a time-saving practical procedure for early detection of the lymphoid cell responses. It would readily lend itself to flow cytofluorimetry for possible routine diagnostic use.

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