Effect of Age, Temperature and Inoculum Size on Non-Infective Vaccinia Virus Yields in Eggs.

Abstract
A relative increase in noninfective or incomplete vaccinia virus occurs during virus growth in the chorioallantoic membrane of 12-day-old eggs incubated at 26[degree]C and 30[degree]C. Vaccinia growth in membranes of eggs 8 and 15 days of age is reduced but no increase in non-infective vaccinia was found. Serial passage of high concentrations of vaccinia virus in 12-day-old eggs likewise failed to increase the proportion of noninfective virus.

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