A deazaadenosine‐insensitive methylation of phosphatidylethanolamine is involved in lipoprotein secretion
- 18 August 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 204 (2) , 243-246
- https://doi.org/10.1016/0014-5793(86)80820-1
Abstract
We have examined the effect of inhibitors of methylation of phosphatidylethanolamine on lipoprotein secretion from cultured rat hepatocytes. The incorporation of [1‐3H]ethanolamine into phosphatidylcholine of hepatocytes and secreted lipoproteins was inhibited by greater than 90% by the methylation inhibitors 3‐deazaadenosine and Neplanocin. In addition, these compounds strongly inhibited the incorporation of [3‐3H]serine into the choline moiety of phosphatidylcholine of the hepatocytes, but had no effect on incorporation of [3‐3H]serine into secreted phosphatidylcholine. The results suggest that a pool of phosphatidylcholine targeted for lipoprotein secretion originates from phosphatidylethanolamine made from serine and this methylation reaction has the unique property of being insensitive to 3‐deazaadenosine.Keywords
This publication has 10 references indexed in Scilit:
- Intracellular sites of lipid synthesis and the biogenesis of mitochondriaPublished by Elsevier ,2021
- Specific pools of phospholipids are used for lipoprotein secretion by cultured rat hepatocytes.Journal of Biological Chemistry, 1986
- The biosynthesis of phosphatidylcholine by methylation of phosphatidylethanolamine derived from ethanolamine is not required for lipoprotein secretion by cultured rat hepatocytesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Phosphatidylserine functions as the major precursor of phosphatidylethanolamine in cultured BHK-21 cells.Proceedings of the National Academy of Sciences, 1984
- Neplanocin A. A potent inhibitor of S-adenosylhomocysteine hydrolase and of vaccinia virus multiplication in mouse L929 cells.Journal of Biological Chemistry, 1984
- Isolation and analysis of lipoproteins secreted by rat liver hepatocytesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Inhibition of phosphatidylethanolamine N-methylation by 3-deazaadenosine stimulates the synthesis of phosphatidylcholine via the CDP-choline pathway.Journal of Biological Chemistry, 1982
- Analogues of cyclic AMP inhibit phosphatidylethanolamine N-methylation by cultured rat hepatocytesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- The base exchange enzymes and phospholipase D of mammalian tissueCanadian Journal of Biochemistry, 1980
- Perturbation of biochemical transmethylations by 3-deazaadenosine in vivoBiochemical Pharmacology, 1979