DEMONSTRATION OF A PYRUVATE DEHYDROGENASE ACTIVATOR IN INSULIN-TREATED HUMAN PLACENTAL PLASMA MEMBRANE
- 31 December 1981
- journal article
- research article
- Published by Biomedical Research Press in Biomedical Research
- Vol. 3 (6) , 599-605
- https://doi.org/10.2220/biomedres.3.599
Abstract
A factor capable of activating pyruvate dehydrogenase (PDH) was found in human placental plasma membrane, which is known to be rich in insulin receptors. This factor was isolated from insulin-treated plasma membrane fraction, and its activity was demonstrated after Sephadex G-25 gel chromatography. The activity of PDH was assayed using mitochondrial fractions from rat adipocytes and hepatocytes. A significant stimulation of PDH activity occurred by addition of the gel filtration fractions of the extract corresponding to 1000-1500 daltons from insulin-treated placental plasma membrane. This activity was acid- and heat-stable but inactivated by treatment with trypsin or chymotrypsin, suggesting that it is a peptide. An insulin-dependent PDH activator, similar to that of adipocytes, exists also in human placenta.This publication has 10 references indexed in Scilit:
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