NreB from Achromobacter xylosoxidans 31A Is a Nickel-Induced Transporter Conferring Nickel Resistance
Open Access
- 1 May 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (9) , 2803-2807
- https://doi.org/10.1128/jb.183.9.2803-2807.2001
Abstract
There are two distinct nickel resistance loci on plasmid pTOM9 fromAchromobacter xylosoxidans 31A, ncc andnre. Expression of the nreB gene was specifically induced by nickel and conferred nickel resistance on bothA. xylosoxidans 31A and Escherichia coli. E. coli cells expressing nreB showed reduced accumulation of Ni2+, suggesting that NreB mediated nickel efflux. The histidine-rich C-terminal region of NreB was not essential but contributed to maximal Ni2+ resistance.Keywords
This publication has 31 references indexed in Scilit:
- A Gene Cluster Involved in Metal Homeostasis in the Cyanobacterium Synechocystis sp. Strain PCC 6803Journal of Bacteriology, 2000
- Regulation of the cnr Cobalt and Nickel Resistance Determinant from Ralstonia sp. Strain CH34Journal of Bacteriology, 2000
- Nickel carcinogenesis, mutation, epigenetics, or selection.Environmental Health Perspectives, 1999
- Inhibitor effects on the accumulation and efflux of nickel ions in plasmid pMOL28-harboring strains of Alcaligenes eutrophusArchiv für Mikrobiologie, 1990
- Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.Proceedings of the National Academy of Sciences, 1989
- A set of cassettes and improved vectors for genetic and biochemical characterization of pseudomonas genesGene, 1987
- The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequencesGene, 1984
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Toxicity of Nickel to Microbes: Environmental AspectsAdvances in applied microbiology, 1983
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982