Analyses of Mono- and Dihydroxybenzenes in Tobacco Smoke and Pyrolyzates by Glass Capillary Gas Chromatography

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Abstract
Gas chromatography (GC) with wall-coated open tubular (WCOT) glass capillary columns has become the most widely used method of analysis for mono- and dihydroxybenzenes or phenols. This paper presents results of investigations in which glass capillary column GC was used for the separation of mono- and dihydroxybenzenes as underivatized compounds and as trimethytsilyl derivatives of phenols occurring in cigarette smoke condensates, tobacco pyrolyzates, or their fractions. Methodology for the routine quantitative analysis of the major components from these samples is presented. Special emphasis was placed on the capillary inlet system and on liquid phase film thicknesses of capillary columns used in this work. The separations of cigarette smoke, pyrolyzate, and a purified phenolic fraction on SP-1000, SE-54, and SP-2250 WCOT columns are given. Considerations pertaining to routine analyses of a multitude of complex smoke samples are discussed.