Phospholipid Metabolism in Vitamin A-deficient Rats

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Abstract
The effect of vitamin A deficiency on the in vivo uptake of 32P-phosphoric acid, 14C-choline, 14C-oleic acid, 14C-palmitic acid and 14C-glycerol by phospholipids from subcellular fractions of rat liver and their mitochondrial membrane fractions was examined. Vitamin A deficiency resulted in an increased incorporation of 14C-choline, 14C-oleic and 14C-palmitic acids into phospholipids from various subcellular fractions. The majority of this increase was found in the phosphatidyl choline component of all subcellular fractions. The uptake of 14C-glycerol into the respective subcellular phospholipids from deficient and control animals was similar. In all instances the specific activity of the mitochondrial phospholipids was highest in the outer membrane fraction. The specific activity of the mitochondrial phospholipids labeled with 32P, 14C-choline, 14C-palmitic and 14C-oleic acids was highest in the outer membrane from deficient animals. The uptake of 14C-glycerol into the respective mitochondrial membrane fractions from control and deficient groups was approximately the same. No significant difference was noted in the fatty acid composition of total phospholipids from control and deficient groups.

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