FLOW CYTOMETRIC ANALYSIS OF MACROPHAGE HETEROGENEITY AND DIFFERENTIATION - UTILIZATION OF ELECTRONIC CELL-VOLUME AND FLUORESCENT SUBSTRATES CORRESPONDING TO COMMON MACROPHAGE MARKERS
- 1 January 1982
- journal article
- research article
- Vol. 32 (4) , 273-285
Abstract
Electronic cell volume (ECV) and several fluorescent analogs of traditional macrophage markers were utilized in a flow cytometric characterization of macrophage heterogeneity. With this technique, various features of macrophage differentiation and activation can be determined on as few as 10,000 cells/assay. Quantitations of cell volume, phagocytosis, RNA, myeloperoxidase, acid phosphatase and the ectoenzyme leucine aminopeptidase were carried out on cultured human monocytes and peritoneal macrophages. Quantitations of the amount of a particular marker on a per cell basis and population distributions are of particular value in characterizing the heterogeneity in resident and inflammatory macrophages and the temporal relationships between different markers during macrophage activation and differentiation. This technique will facilitate a sophisticated analysis of monocyte-macrophage development by permitting the simultaneous analysis of a number of these features. In this way the interrelationships between different macrophage markers can be quantitated at the single cell level.This publication has 2 references indexed in Scilit:
- Macrophage heterogeneity in tumor resistance: Cytostatic and cytotoxic activity of corynebacterium parvum-activated and proteose peptone-elicited rat macrophages against Moloney sarcoma tumor cellsCellular Immunology, 1980
- INTRACELLULAR LEVELS AND STIMULATED RELEASE OF LYSOSOMAL-ENZYMES FROM HUMAN PERIPHERAL-BLOOD MONOCYTES AND MONOCYTE-DERIVED MACROPHAGES1980