Uninterrupted DNA synthesis during S-phase in untransformed diploid hamster fibroblasts

1 February 1985

journal article
research article
Published by Springer Nature in Chromosoma

Vol. 91 (2) , 164-166
https://doi.org/10.1007/bf00294061

Abstract

Untransformed Syrian hamster fibroblasts in exponential growth were exposed to a pulse of [³H]-thymidine for 5 min, followed immediately by bromodeoxyuridine, and serial samples were taken up to 16 h. Preparations were autoradiographed and stained for replication banding. No cell with replication bands was found without significant [³H]-thymidine uptake, although the extent of uptake varied between sub-phases of S. Thus there is no indication of a total cessation of synthesis at any period during S-phase.

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